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Image Search Results
Journal: Frontiers in Cellular and Infection Microbiology
Article Title: Low Density Lipoprotein Receptor-Related Protein-1 (LRP1) Is Involved in the Uptake of Clostridioides difficile Toxin A and Serves as an Internalizing Receptor
doi: 10.3389/fcimb.2020.565465
Figure Lengend Snippet: Binding of TcdA and TcdB to the cell surface. (A) Binding of TcdA and TcdB to LRP1 -/- mouse embryonic fibroblasts (MEFs) and LRP1 +/+ MEFs was allowed at 4°C for 1 h . Unbound toxin was removed by washing with buffer. Cells with bound toxins were lysed and cell surface bound toxins were detected by immunoblotting. (B) Bound TcdA and TcdB were quantified using LabImage 1D software. Values are the mean ± SD from three independent experiments. (C) LRP1 cluster II and IV and lysates from LRP1 +/+ MEFs were immobilized on nitrocellulose and exposed to solutions of TcdA (3 nM) or TcdB (3 nM) for 1 h. Bound toxin was detected using toxin specific antitoxin antibodies. * indicates significant difference, p < 0,05 as analyzed using student´s t-test.
Article Snippet: The signals were detected with the INTAS Chemo Cam Imager (Intas Science Imaging Instruments GmbH, Göttingen, Germany) and analyzed densitometrically using the
Techniques: Binding Assay, Western Blot, Software